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प्रश्न
Ti plasmid being used for introducing genes in plants obtained from ______.
विकल्प
Agrobacterium rhizogenes
Escherichia coli
Agrobacterium T20
Agrobacterium tumefaciens
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उत्तर
Ti plasmid being used for introducing genes in plants obtained from Agrobacterium tumefaciens.
संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
The bacterium which causes a plant disease called crown gall is _______________.
The technique which involves addition or deletion of genes is ______.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
The source of taq Polymerase used in PCR is a ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
The PCR technique is used for ______.
Which vector is mostly used in rDNA technology in plants?
What is polymerase chain reaction (PCR)?
