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प्रश्न
Who among the following was awarded the Nobel Prize for the development of PCR technique?
विकल्प
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
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उत्तर
Kary Mullis
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Distinguish between RNA and DNA.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
The enzyme restriction endonuclease ______.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
____________ cuts the DNA within the specific positions.
A hind of biotechnology involving manipulation of DNA is ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Expand the following acronym:
BAC
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
______technique is used for in vitro gene cloning or gene multiplication.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Which amino acid is coded by the initiation codon?
Name the enzymes that are called molecular scissors.
Give a reason for the following:
DNA cannot enter directly into the host cell.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
