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प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
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उत्तर
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संबंधित प्रश्न
The technique which involves addition or deletion of genes is ______.
From the following which one is NOT a feature of the plasmids?
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
______ is a Type I restriction enzyme.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Polymerase Chain Reaction is made possible by ____________.
Lytic cycle involves bacteriophage and ____________.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
What is true for plasmid?
Nucleic acid segment tagged with a radioactive molecule is called ______.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
How does EcoRI differ from an exonuclease?
Name the enzymes that are called molecular scissors.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
Genetic engineering is also known as:
