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प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
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उत्तर
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
Name the most commonly used bioreactor and describe its working.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
ECoRI is obtained from ______.
Polymerase chain reaction is most useful in ______.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
The polymerase chain reaction is a powerful technique to ______.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
