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प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
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उत्तर
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संबंधित प्रश्न
Write a short note on Electrophoresis.
What is the cell that receives a recombinant gene called?
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following DNA sequence is recognised and cut by EcoR I?
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Polymerase Chain Reaction is made possible by ____________.
EcoRl & Hind III are the examples of ______
Which of the following viruses has the capacity to produce lysozymes?
Which one is a true statement regarding DNA polymerase used in PCR?
Which of the following is not required in the preparation of a recombinant DNA molecule?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
What is meant by gene cloning?
Annealing step of PCR operates at ______ °C.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Genetic engineering is also known as:
