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प्रश्न
Write a short note on Electrophoresis.
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उत्तर
It is a technique commonly used in the lab to separate charged molecules like DNA, according to size.
There are two types of gels used for electrophoresis.
– Agarose gel for separation of medium and large-sized nucleic acids
– Polyacrylamide gels for separation of proteins and purification of enzymes or separation of DNA fragments.
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संबंधित प्रश्न
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
The gene transfer between unrelated plants is brought about by ____________.
EcoRl & Hind III are the examples of ______
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Name and describe the technique that helps in separation of DNA fragments.
Plasmid is ______.
Write steps involved in gene amplification.
Write a short note on vectors.
