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प्रश्न
Write a short note on Electrophoresis.
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उत्तर
It is a technique commonly used in the lab to separate charged molecules like DNA, according to size.
There are two types of gels used for electrophoresis.
– Agarose gel for separation of medium and large-sized nucleic acids
– Polyacrylamide gels for separation of proteins and purification of enzymes or separation of DNA fragments.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
What is gene cloning? Explain different tools used for it.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Which of the following viruses has the capacity to produce lysozymes?
Annealing step of PCR operates at ______ °C.
To be a cloning vector, a plasmid require ______.
Explain the mechanism of PCR.
Which one of the following enzymes is used to join DNA fragments?
