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प्रश्न
Write a short note on Electrophoresis.
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उत्तर
It is a technique commonly used in the lab to separate charged molecules like DNA, according to size.
There are two types of gels used for electrophoresis.
– Agarose gel for separation of medium and large-sized nucleic acids
– Polyacrylamide gels for separation of proteins and purification of enzymes or separation of DNA fragments.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Define biotechnology? Which are the basic principles and process of biotechnology?
Identify the CORRECT sequence of steps in PCR.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
The PCR technique is used for ______.
Give a reason for the following:
DNA cannot enter directly into the host cell.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
