Advertisements
Advertisements
प्रश्न
Give a reason for the following:
DNA cannot enter directly into the host cell.
Advertisements
उत्तर
- DNA is a hydrophilic molecule that is negatively charged. The cell membrane (plasma membrane) is hydrophobic.
- It is mostly composed of lipids, which prevent hydrophilic molecules like DNA from crossing the cell membrane.
संबंधित प्रश्न
What are plasmids?
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Which one is a true statement regarding DNA polymerase used in PCR?
Which vector is mostly used in rDNA technology in plants?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Name the enzymes that are called molecular scissors.
How many sets of primers are required in each cycle of PCR?
What is the primary focus of genetic engineering?
