English

Give a reason for the following: DNA cannot enter directly into the host cell. - Biology (Theory)

Advertisements
Advertisements

Question

Give a reason for the following:

DNA cannot enter directly into the host cell.

Short/Brief Note
Advertisements

Solution

  1. DNA is a hydrophilic molecule that is negatively charged. The cell membrane (plasma membrane) is hydrophobic.
  2. It is mostly composed of lipids, which prevent hydrophilic molecules like DNA from crossing the cell membrane.
shaalaa.com
  Is there an error in this question or solution?
2022-2023 (March) Official

Video TutorialsVIEW ALL [1]

RELATED QUESTIONS

State the role of DNA ligase in biotechnology.


____________ cuts the DNA within the specific positions.


EcoRl & Hind III are the examples of ______ 


The source of taq Polymerase used in PCR is a ______.


Who among the following was awarded the Nobel Prize for the development of PCR technique?


Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:

  1. Name the host for this cloning vector.
  2. Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
  3. Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
    5' --- GTACGAATCCTGA --- 3'
    3' --- CATGCTTAGGACT --- 3'

Which of the following is the correct recognition sequence of restriction enzyme Eco RI?


Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:

5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'

  1. Name a restriction enzyme which can recognise this DNA sequence.
  2. Write the sequence after digestion.
  3. Why are the ends generated after digestion called sticky ends?

Which amino acid is coded by the initiation codon?


Name the source of thermostable DNA polymerase.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×