Advertisements
Advertisements
प्रश्न
Give a reason for the following:
DNA cannot enter directly into the host cell.
Advertisements
उत्तर
- DNA is a hydrophilic molecule that is negatively charged. The cell membrane (plasma membrane) is hydrophobic.
- It is mostly composed of lipids, which prevent hydrophilic molecules like DNA from crossing the cell membrane.
APPEARS IN
संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
The time taken to complete one cycle of PCR is around ______.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Which molecules are called molecular scissors? Why are they called so?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
The polymerase chain reaction is a powerful technique to ______.
What is EcoRI?
The three basic steps in genetic modification are:
