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प्रश्न
Write a short note on Electrophoresis.
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उत्तर
It is a technique commonly used in the lab to separate charged molecules like DNA, according to size.
There are two types of gels used for electrophoresis.
– Agarose gel for separation of medium and large-sized nucleic acids
– Polyacrylamide gels for separation of proteins and purification of enzymes or separation of DNA fragments.
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संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
What are the restriction endonucleases? Give the rules of their nomenclature.
The source of taq Polymerase used in PCR is a ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Which vector is mostly used in rDNA technology in plants?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
What is EcoRI?
Importance of PCR.
In vitro amplification of DNA segments is called______.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
