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प्रश्न
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
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उत्तर
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
The bacterium which causes a plant disease called crown gall is _______________.
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Explain the properties of a good or ideal cloning vector for rDNA technology.
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Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
From the following which one is NOT a feature of the plasmids?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Polymerase Chain Reaction is made possible by ____________.
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
A hind of biotechnology involving manipulation of DNA is ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
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The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Which amino acid is coded by the initiation codon?
Name the enzymes that are called molecular scissors.
How many sets of primers are required in each cycle of PCR?
Which one of the following enzymes is used to join DNA fragments?
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
