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प्रश्न
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
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उत्तर
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
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संबंधित प्रश्न
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| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
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Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.




