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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

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प्रश्न

While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

अति संक्षिप्त उत्तर
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उत्तर

If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.

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अध्याय 11: Biotechnology : Principles and Processes - VERY SHORT ANSWER [पृष्ठ ७८]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
VERY SHORT ANSWER | Q 7. | पृष्ठ ७८
नूतन Biology [English] Class 12 ISC
अध्याय 11 Principles and Processes of Biotechnology
HIGHER ORDER THINKING SKILLS QUESTIONS (HOTS) | Q 4. | पृष्ठ ५३४

संबंधित प्रश्न

Name two commonly used bioreactors.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Downstream processing


Explain briefly:

PCR


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?


Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?


In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?


Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?


Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?


Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?


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