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प्रश्न
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
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उत्तर
If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.
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संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
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Bioreactors
Explain briefly:
PCR
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A single strand of nucleic acid tagged with a radioactive molecule is called ______.
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Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
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Reason (R): The primers bind to the double stranded DNA at their complementary regions.
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