Advertisements
Advertisements
प्रश्न
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Advertisements
उत्तर
If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.
APPEARS IN
संबंधित प्रश्न
Name two commonly used bioreactors.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Explain briefly:
Chitinase
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
______ plays an important role in blood clotting.
PCR and restriction fragment length polymorphism are the methods for ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
An antibiotic resistance gene in a vector usually helps in the selection of ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Which of the following statements is incorrect?
