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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Can you recall meiosis and indicate at what stage recombinant DNA is made?

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प्रश्न

Can you recall meiosis and indicate at what stage recombinant DNA is made?

अति संक्षिप्त उत्तर
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उत्तर

Recombinant DNA is formed due to crossing over between non-sister chromatids of a homologous chromosome. It occurs during the pachytene stage of prophase of meiosis I.

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अध्याय 9: Biotechnology : Principles and Processes - EXERCISES [पृष्ठ १७५]

APPEARS IN

एनसीईआरटी Biology [English] Class 12
अध्याय 9 Biotechnology : Principles and Processes
EXERCISES | Q 8. | पृष्ठ १७५
नूतन Biology [English] Class 12 ISC
अध्याय 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 7. | पृष्ठ ५३५

संबंधित प्रश्न

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Describe briefly the following:

Downstream processing


Explain briefly:

PCR


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


Which of the following term used for defining a viral genome incorporated into host DNA?


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


Gene Amplification using primers can be done by ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


In PCR, primers are used for ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?


How is copy number of the plasmid vector related to yield of recombinant protein?


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.


Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?


In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?


Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?


How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?


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