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प्रश्न
Can you recall meiosis and indicate at what stage recombinant DNA is made?
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उत्तर
Recombinant DNA is formed due to crossing over between non-sister chromatids of a homologous chromosome. It occurs during the pachytene stage of prophase of meiosis I.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Explain briefly:
PCR
State the importance of using a bioreactor.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Which of the following statements is incorrect?
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
