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प्रश्न
State the importance of using a bioreactor.
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उत्तर
The importance of using bioreactors is as follows:
(1) It provides large volume for cultures. Thus, products are obtained in high quantity.
(2) It provides optimal conditions like temperature and pH for growth of desired product.
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संबंधित प्रश्न
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
State the advantage of using Thermostable DNA polymerase.
Which of the following statements is incorrect?
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
