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प्रश्न
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
विकल्प
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
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उत्तर
Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.
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संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Explain briefly:
Chitinase
Which of the following term used for defining a viral genome incorporated into host DNA?
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
The first step in recombinant DNA technology is
An antibiotic resistance gene in a vector usually helps in the selection of ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
