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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Significance of 'heat shock' method in bacterial transformation is to facilitate ______.

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प्रश्न

Significance of 'heat shock' method in bacterial transformation is to facilitate ______.

विकल्प

  • Binding of DNA to the cell wall

  • Uptake of DNA through membrane transport proteins

  • Uptake of DNA through transient pores in the bacterial cell wall

  • Expression of antibiotic resistance gene

MCQ
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उत्तर

Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.

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अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 12. | पृष्ठ ७७

संबंधित प्रश्न

Name two commonly used bioreactors.


Explain briefly:

Chitinase


State the importance of using a bioreactor.


______ plays an important role in blood clotting.


PCR and restriction fragment length polymorphism are the methods for ______.


The first step in recombinant DNA technology is


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Rising of dough is due to ______.


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.


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