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प्रश्न
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
विकल्प
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
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उत्तर
Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.
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संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Describe briefly the following:
Downstream processing
Explain briefly:
Chitinase
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
PCR and restriction fragment length polymorphism are the methods for ______.
Gene Amplification using primers can be done by ______.
In PCR, primers are used for ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
How is copy number of the plasmid vector related to yield of recombinant protein?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
