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प्रश्न
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
विकल्प
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
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उत्तर
Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Downstream processing
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
PCR and restriction fragment length polymorphism are the methods for ______.
The first step in recombinant DNA technology is
In PCR, primers are used for ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
Which series of operations is collectively referred to as 'downstream processing'?
