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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

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प्रश्न

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

आकृति
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उत्तर

A - Denaturation

B - Annealing

C - Extension of primers

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अध्याय 11: Biotechnology : Principles and Processes - SHORT ANSWER [पृष्ठ ८०]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 11. | पृष्ठ ८०

संबंधित प्रश्न

Name two commonly used bioreactors.


Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Describe briefly the following:

Bioreactors


Describe briefly the following:

Downstream processing


Explain briefly:

PCR


Explain briefly:

Chitinase


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


Which of the following term used for defining a viral genome incorporated into host DNA?


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


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