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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

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प्रश्न

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

आकृति
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उत्तर

A - Denaturation

B - Annealing

C - Extension of primers

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अध्याय 11: Biotechnology : Principles and Processes - SHORT ANSWER [पृष्ठ ८०]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 11. | पृष्ठ ८०

संबंधित प्रश्न

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Describe briefly the following:

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During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


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Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Which of the following statements is incorrect?


Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?


Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?


How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?


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