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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

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प्रश्न

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

आकृति
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उत्तर

A - Denaturation

B - Annealing

C - Extension of primers

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अध्याय 11: Biotechnology : Principles and Processes - SHORT ANSWER [पृष्ठ ८०]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 11. | पृष्ठ ८०

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During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


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Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
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Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

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