Advertisements
Advertisements
प्रश्न
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Advertisements
उत्तर
A - Denaturation
B - Annealing
C - Extension of primers
APPEARS IN
संबंधित प्रश्न
Name two commonly used bioreactors.
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Downstream processing
State the importance of using a bioreactor.
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
How is copy number of the plasmid vector related to yield of recombinant protein?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Which of the following statements is incorrect?
