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प्रश्न
Name the regions marked A, B and C.
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उत्तर
A - Bam HI
B - Pst I
C - Ampicillin resistance gene (ampR)
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following is correctly matched?
Which of the following is not a genetic vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What is the primary function of a vector in recombinant DNA technology?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
