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प्रश्न
Name the regions marked A, B and C.
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उत्तर
A - Bam HI
B - Pst I
C - Ampicillin resistance gene (ampR)
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Write the role of ‘restriction sites’ in the cloning vector pBR322.
The genetic material of virus includes:
A clone is a ______.
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
