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प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
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उत्तर
It is the recognition site for restriction enzymes (e.g., EcoRI, Hind III, Pvul, BamHI). Recognition sites are the genetic sequences to which restriction enzymes bind and cut DNA.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
The genetic material of virus includes:
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
