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प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
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उत्तर
It is the recognition site for restriction enzymes (e.g., EcoRI, Hind III, Pvul, BamHI). Recognition sites are the genetic sequences to which restriction enzymes bind and cut DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
What is ‘Ori’ ? State its importance during cloning of a vector.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
