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प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
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उत्तर
It is the recognition site for restriction enzymes (e.g., EcoRI, Hind III, Pvul, BamHI). Recognition sites are the genetic sequences to which restriction enzymes bind and cut DNA.
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Describe the role of CaCl2 in the preparation of competent cells?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
