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प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
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उत्तर
It is the recognition site for restriction enzymes (e.g., EcoRI, Hind III, Pvul, BamHI). Recognition sites are the genetic sequences to which restriction enzymes bind and cut DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
A clone is a ______.
Which of the following is correctly matched?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
Which of the following describes the function of a selectable marker in a cloning vector?
What is the primary role of a cloning site in a vector?
