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प्रश्न
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
विकल्प
Medium with Tetracycline Medium with Ampicillin Growth No growth Medium with Tetracycline Medium with Ampicillin No growth Growth Medium with Tetracycline Medium with Ampicillin No growth No growth Medium with Tetracycline Medium with Ampicillin Growth Growth
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उत्तर
| Medium with Tetracycline | Medium with Ampicillin |
| No growth | Growth |
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संबंधित प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Which of the following is not a cloning vector?
