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प्रश्न
Describe briefly the following:
Origin of replication
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उत्तर
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
The genetic material of virus includes:
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
