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प्रश्न
Describe briefly the following:
Origin of replication
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उत्तर
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Which of the following is not a cloning vector?
What is the primary function of a vector in recombinant DNA technology?
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
Which of the following is a group of vectors used for gene transfer into animals?
