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प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
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उत्तर
pBR322
The Restriction sites mentioned in the figure are Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I (any two can be considered) Antibiotic resistant genes: ampR and tetR
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संबंधित प्रश्न
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
The genetic material of virus includes:
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
