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प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
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उत्तर
pBR322
The Restriction sites mentioned in the figure are Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I (any two can be considered) Antibiotic resistant genes: ampR and tetR
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संबंधित प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
A clone is a ______.
A suitable vector for gene cloning in higher organism is ______.
What does ‘competent’ refer to in competent cells used in transformation experiments?
Name the regions marked A, B and C.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the primary role of a cloning site in a vector?
Which of the following is a group of vectors used for gene transfer into animals?
