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प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
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उत्तर
pBR322
The Restriction sites mentioned in the figure are Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I (any two can be considered) Antibiotic resistant genes: ampR and tetR
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Write the role of ‘restriction sites’ in the cloning vector pBR322.
A clone is a ______.
Which of the following is correctly matched?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
