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प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
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उत्तर
pBR322
The Restriction sites mentioned in the figure are Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I (any two can be considered) Antibiotic resistant genes: ampR and tetR
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
