मराठी
कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

Advertisements
Advertisements

प्रश्न

What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

टीपा लिहा
Advertisements

उत्तर

T-DNA is the only essential part required to make Ti plasmid a cloning vector. The plasmid is disarmed by deleting the tumour-inducing genes in the plasmid so that it becomes an effective cloning vector and remove it harmful effect.

shaalaa.com
  या प्रश्नात किंवा उत्तरात काही त्रुटी आहे का?
पाठ 11: Biotechnology : Principles and Processes - VERY SHORT ANSWER [पृष्ठ ७९]

APPEARS IN

एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
VERY SHORT ANSWER | Q 10. | पृष्ठ ७९

संबंधित प्रश्‍न

Draw a schematic sketch of pBR 322 plasmid and label the following in it:

(a) Any two restriction sites.

(b) Ori and rop genes.

(c) An antibiotic resistant gene.


Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?


Write the role of ‘restriction sites’ in the cloning vector pBR322.


Explain the importance of ‘Selectable marker’, with the help of a suitable example.


Answer the following question.
Expand ‘YAC’ and mention what it was used for.


The genetic material of virus includes:


The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.


A clone is a ______.


Plasmids are suitable vectors for gene cloning because these ______.


Which of the following is not a genetic vector?


Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.


A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?


Describe the role of Agrobacterium tumefaciens in transforming a plant cell.


What is the primary function of a vector in recombinant DNA technology?


What is the basic biological nature of plasmids when used as cloning vectors?


What is the primary role of a cloning site in a vector?


Which of the following is a group of vectors used for gene transfer into animals?


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×