Advertisements
Advertisements
प्रश्न
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Advertisements
उत्तर
No, biomolecules like DNA and protein cannot exhibit biological activity in anhydrous conditions. Hence, life is unsustainable without water.
APPEARS IN
संबंधित प्रश्न
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR and restriction fragment length polymorphism are the methods for ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Rising of dough is due to ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Which of the following statements is incorrect?
Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
Which series of operations is collectively referred to as 'downstream processing'?
