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प्रश्न
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
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उत्तर
No, biomolecules like DNA and protein cannot exhibit biological activity in anhydrous conditions. Hence, life is unsustainable without water.
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Explain briefly:
PCR
Explain briefly:
Chitinase
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR and restriction fragment length polymorphism are the methods for ______.
Gene Amplification using primers can be done by ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Rising of dough is due to ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
State the advantage of using Thermostable DNA polymerase.
