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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
What are plasmids?
Give the applications of the PCR technique.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
In vitro amplification of DNA or RNA segment is known as ___________.
Explain the properties of a good or ideal cloning vector for rDNA technology.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
What is gene cloning? Explain different tools used for it.
Explain types of enzymes used in biotechnology?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
The gene transfer between unrelated plants is brought about by ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Which one is a true statement regarding DNA polymerase used in PCR?
A hind of biotechnology involving manipulation of DNA is ______.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Name the plant disease caused by Agrobacterium tumefaciens.
The DNA polymerase enzyme used in PCR is obtained from______.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Importance of PCR.
How many sets of primers are required in each cycle of PCR?
Which one of the following enzymes is used to join DNA fragments?
