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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Distinguish between plasmid DNA and chromosomal DNA.
How has the development of bioreactor helped in biotechnology?
Name the most commonly used bioreactor and describe its working.
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
In vitro amplification of DNA or RNA segment is known as ___________.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
From the following which one is NOT a feature of the plasmids?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the CORRECT sequence of steps in PCR.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
____________ cuts the DNA within the specific positions.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Which of the following statements is correct with respect to cellular respiration?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Which of these are most widely used in genetic engineering?
The DNA polymerase enzyme used in PCR is obtained from______.
What are the different types of electrophoresis?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Give a reason for the following:
DNA cannot enter directly into the host cell.
