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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
How has the development of bioreactor helped in biotechnology?
Name the most commonly used bioreactor and describe its working.
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Why are bio-fertilizers preferred over chemical fertilizers?
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Enlist and write in brief about the different biological tools required in rDNA technology.
The technique which involves addition or deletion of genes is ______.
ECoRI is obtained from ______.
What is plasmid?
Alu-I is obtained from which organism?
What is the role of Taq-polymerase in PCR technology?
What is Recognition sequence? Explain in brief.
From the following which one is NOT a feature of the plasmids?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
____________ cuts the DNA within the specific positions.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Name and describe the technique that helps in separation of DNA fragments.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Restriction enzymes were discovered by ______.
Which of these are most widely used in genetic engineering?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Write a short note on vectors.
The three basic steps in genetic modification are:
Which of the following best describes the relationship between genetic engineering and recombinant DNA?
