Advertisements
Advertisements
प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Advertisements
उत्तर
Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. Stanley Cohen and Herbert Boyer accomplished this in 1972.
APPEARS IN
संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
Enlist and write in brief about the different biological tools required in rDNA technology.
The technique which involves addition or deletion of genes is ______.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
What is true for plasmid?
Name a recombinant vaccine that is currently being used in vaccination programme.
Restriction enzymes were discovered by ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write a short note on vectors.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
