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प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
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उत्तर
Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. Stanley Cohen and Herbert Boyer accomplished this in 1972.
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संबंधित प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
What is the cell that receives a recombinant gene called?
What is PCR? Explain different steps involved in it.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Restriction enzymes were discovered by ______.
What are the different types of electrophoresis?
Which amino acid is coded by the initiation codon?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
