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प्रश्न
Name the source of thermostable DNA polymerase.
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उत्तर
A bacterium Thermus aquaticus.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Polymerase chain reaction is most useful in ______.
Identify the CORRECT sequence of steps in PCR.
Which of the following statements is correct with respect to cellular respiration?
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Plasmid is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write a short note on vectors.
