Advertisements
Advertisements
प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
Advertisements
उत्तर
Taq polymerase is an enzyme obtained from Thermus aquaticus which is a bacterium that normally lives in hot water springs. It is used in amplifying small quantities of short segments of DNA by polymerase chain reaction.
APPEARS IN
संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
The technique which involves addition or deletion of genes is ______.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Which of the following DNA sequence is recognised and cut by EcoR I?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
To be a cloning vector, a plasmid require ______.
