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प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
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उत्तर
Taq polymerase is an enzyme obtained from Thermus aquaticus which is a bacterium that normally lives in hot water springs. It is used in amplifying small quantities of short segments of DNA by polymerase chain reaction.
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संबंधित प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
