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प्रश्न
What is Recognition sequence? Explain in brief.
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उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
A hind of biotechnology involving manipulation of DNA is ______.
Describe three steps involved in mechanism of PCR.
What is EcoRI?
Expand YAC used in the field of Biotechnology.
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
DNA cannot enter directly into the host cell.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
