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प्रश्न
What is Recognition sequence? Explain in brief.
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उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
Expand the following abbreviation: BAC
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Which molecules are called molecular scissors? Why are they called so?
Which of the following viruses has the capacity to produce lysozymes?
What is meant by gene cloning?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Which vector is mostly used in rDNA technology in plants?
How does EcoRI differ from an exonuclease?
Expand YAC used in the field of Biotechnology.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
