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प्रश्न
What is Recognition sequence? Explain in brief.
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उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Expand the following abbreviation: BAC
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Name a recombinant vaccine that is currently being used in vaccination programme.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Expand the following acronym:
BAC
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
