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प्रश्न
What is Recognition sequence? Explain in brief.
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उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
What is plasmid?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Which of these are most widely used in genetic engineering?
Importance of PCR.
Name the source of thermostable DNA polymerase.
What is the primary focus of genetic engineering?
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
