Advertisements
Advertisements
प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
Advertisements
उत्तर
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
APPEARS IN
संबंधित प्रश्न
Alu-I is obtained from which organism?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
The gene transfer between unrelated plants is brought about by ____________.
Human insulin is produced by culturing the genetically engineered ______
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
To be a cloning vector, a plasmid require ______.
What is EcoRI?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
