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प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
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उत्तर
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
In vitro amplification of DNA or RNA segment is known as ___________.
Polymerase chain reaction is most useful in ______.
What is PCR? Explain different steps involved in it.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Name the enzymes that are called molecular scissors.
Which one of the following enzymes is used to join DNA fragments?
