Advertisements
Advertisements
प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Advertisements
उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
संबंधित प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Describe two vectors less methods of gene transfer used in rDNA technology.
Why are bio-fertilizers preferred over chemical fertilizers?
In vitro amplification of DNA or RNA segment is known as ___________.
From the following which one is NOT a feature of the plasmids?
From the following identify the methods of electrophoresis.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Polymerase Chain Reaction is made possible by ____________.
The vector Baculovirus is used for gene transfer in ____________.
Human insulin is produced by culturing the genetically engineered ______
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Nucleic acid segment tagged with a radioactive molecule is called ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Expand the following acronym:
BAC
The DNA polymerase enzyme used in PCR is obtained from______.
Write steps involved in gene amplification.
Which amino acid is coded by the initiation codon?
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
