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प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
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उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
संबंधित प्रश्न
Give an account of the Blue-White Method of selection of recombinants.
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Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
ECoRI is obtained from ______.
What is plasmid?
Which of the following is TRUE regarding EcoR I?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
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From the following identify the methods of electrophoresis.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
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Which of the following is not required in the preparation of a recombinant DNA molecule?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Expand the following acronym:
BAC
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
