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प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
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उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
संबंधित प्रश्न
Distinguish between RNA and DNA.
Give an account of the Blue-White Method of selection of recombinants.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Describe two vectors less methods of gene transfer used in rDNA technology.
The bacterium which causes a plant disease called crown gall is _______________.
Explain the properties of a good or ideal cloning vector for rDNA technology.
What is the role of Taq-polymerase in PCR technology?
Explain types of enzymes used in biotechnology?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
The time taken to complete one cycle of PCR is around ______.
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following statements is correct with respect to cellular respiration?
A hind of biotechnology involving manipulation of DNA is ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Name the enzymes that are called molecular scissors.
Give a reason for the following:
DNA cannot enter directly into the host cell.
