Advertisements
Advertisements
प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Advertisements
उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Distinguish between RNA and DNA.
How has the development of bioreactor helped in biotechnology?
Expand the following abbreviation: BAC
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
In vitro amplification of DNA or RNA segment is known as ___________.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Which of the following is TRUE regarding EcoR I?
Which of the following DNA sequence is recognised and cut by EcoR I?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Polymerase Chain Reaction is made possible by ____________.
Lytic cycle involves bacteriophage and ____________.
Which molecules are called molecular scissors? Why are they called so?
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
______technique is used for in vitro gene cloning or gene multiplication.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
The restriction enzymes used as molecular scissors are type of ______.
