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प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
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उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
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| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
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BAC
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