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प्रश्न
Define biotechnology? Which are the basic principles and process of biotechnology?
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उत्तर
Definition:
Biotechnology refers to the development and utilization of biological forms, products or processes for obtaining maximum benefits to man and other forms of life.
- Biotechnology is based on the principles of chemical engineering, genetic engineering and adequate maintenance of sterile conditions.
- Following are the processes used in biotechnology:
- Repairing of the defective genes or replacing of defective genes by healthy genes or normal genes;
- Artificially synthesizing of a totally new gene
संबंधित प्रश्न
Give the applications of the PCR technique.
The vector Baculovirus is used for gene transfer in ____________.
EcoRl & Hind III are the examples of ______
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Name the plant disease caused by Agrobacterium tumefaciens.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The polymerase chain reaction is a powerful technique to ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
What is the importance of PCR.
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
