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प्रश्न
Define biotechnology? Which are the basic principles and process of biotechnology?
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उत्तर
Definition:
Biotechnology refers to the development and utilization of biological forms, products or processes for obtaining maximum benefits to man and other forms of life.
- Biotechnology is based on the principles of chemical engineering, genetic engineering and adequate maintenance of sterile conditions.
- Following are the processes used in biotechnology:
- Repairing of the defective genes or replacing of defective genes by healthy genes or normal genes;
- Artificially synthesizing of a totally new gene
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
From the following identify the methods of electrophoresis.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
______ is a Type I restriction enzyme.
Polymerase Chain Reaction is made possible by ____________.
What is true for plasmid?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
What is EcoRI?
Importance of PCR.
