Advertisements
Advertisements
प्रश्न
Define biotechnology? Which are the basic principles and process of biotechnology?
Advertisements
उत्तर
Definition:
Biotechnology refers to the development and utilization of biological forms, products or processes for obtaining maximum benefits to man and other forms of life.
- Biotechnology is based on the principles of chemical engineering, genetic engineering and adequate maintenance of sterile conditions.
- Following are the processes used in biotechnology:
- Repairing of the defective genes or replacing of defective genes by healthy genes or normal genes;
- Artificially synthesizing of a totally new gene
संबंधित प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Which of the following is an INCORRECT match?
Which molecules are called molecular scissors? Why are they called so?
Which one is a true statement regarding DNA polymerase used in PCR?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Importance of PCR.
