Advertisements
Advertisements
प्रश्न
What would be the molar concentration of human DNA in a human cell?
Advertisements
उत्तर
Molar concentration is the number of moles of solute in a solution divided by its volume, given in litres.
The average weight of a DNA base pair = 650 daltons (1 dalton equals the mass of a single hydrogen atom or 1.67 × 10−24 grams)
The molecular weight of a double-stranded DNA molecule = Total no. of base pairs × 650 daltons.
The human genome is 3.3 × 109 base pairs in length.
Hence, the weight of the human genome
= 3.3 × 109 base pair × 650 Daltons
= 3.59 × 10−12 gm
The molar concentration of DNA can be calculated accordingly.
संबंधित प्रश्न
What are plasmids?
Write a short note on Electrophoresis.
The bacterium which causes a plant disease called crown gall is _______________.
Polymerase chain reaction is most useful in ______.
Define vector? write any two examples,
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following device is required for PCR?
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The vector Baculovirus is used for gene transfer in ____________.
EcoRl & Hind III are the examples of ______
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
The PCR technique is used for ______.
Describe three steps involved in mechanism of PCR.
Explain the mechanism of PCR.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
