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प्रश्न
The biological scissor is ______.
पर्याय
restriction endonuclease
gyrase
DNA ligase
helicase
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उत्तर
The biological scissor is restriction endonuclease.
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संबंधित प्रश्न
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Which of these are most widely used in genetic engineering?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The DNA polymerase enzyme used in PCR is obtained from______.
Write a short note on vectors.
Expand YAC used in the field of Biotechnology.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
