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प्रश्न
The biological scissor is ______.
पर्याय
restriction endonuclease
gyrase
DNA ligase
helicase
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उत्तर
The biological scissor is restriction endonuclease.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Name the most commonly used bioreactor and describe its working.
____________ cuts the DNA within the specific positions.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Name and describe the technique that helps in separation of DNA fragments.
What is true for plasmid?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Describe three steps involved in mechanism of PCR.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
