Advertisements
Advertisements
प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Advertisements
उत्तर
Desirable characteristics of an ideal cloning vector:
- Origin of replication: It is a specific sequence of nucleotides that serves as a binding site for initiator protein (Helicase) to start replication. Any foreign DNA linked with vector DNA also gets replicated along with it, hence it is important for maintaining the copy number of foreign DNA.
- Selectable markers: Cloning vectors must contain at least one selectable marker or it helps in:
– detection of transformed host cells,
– Identification and elimination of nontransformed post cells,
– Isolation and growth of transformed host cells. - Cloning sites: A cloning site is a restriction endonuclease recognition site or target site in vector DNA. It has a palindromic sequence of nitrogenous bases cut by a specific restriction endonuclease enzyme for insertion and linking of foreign DNA.
- Small size: A vector should be small in size because large molecules bind is break up during isolation and purification.
संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Give an example of the source of thermostable enzyme DNA polymerase.
What is Palindromic sequence?
What is Recognition sequence? Explain in brief.
From the following which one is NOT a feature of the plasmids?
Human insulin is produced by culturing the genetically engineered ______
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
What are the different types of electrophoresis?
In vitro amplification of DNA segments is called______.
