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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease are then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is Palindromic sequence?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
EcoRl & Hind III are the examples of ______
The polymerase chain reaction is a powerful technique to ______.
Name the source of thermostable DNA polymerase.
