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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease are then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
Write a short note on Electrophoresis.
Describe two vectors less methods of gene transfer used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Enlist and write in brief about the different biological tools required in rDNA technology.
Polymerase chain reaction is most useful in ______.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Human insulin is produced by culturing the genetically engineered ______
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
