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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease are then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
Describe two vectors less methods of gene transfer used in rDNA technology.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is Recognition sequence? Explain in brief.
EcoRl & Hind III are the examples of ______
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Expand the following acronym:
BAC
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
