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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease are then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
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- A highly active promoter.
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Name the enzymes that are called molecular scissors.
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