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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease are then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
Identify the CORRECT sequence of steps in PCR.
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Write a short note on vectors.
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