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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
विकल्प
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
APPEARS IN
संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is Recognition sequence? Explain in brief.
Identify the characterization techniques that are based on molecular weights of compounds.
From the following identify the methods of electrophoresis.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
DNA fingerprinting refers to ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Name the plant disease caused by Agrobacterium tumefaciens.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
How gene amplification is done?
How does EcoRI differ from an exonuclease?
Explain the mechanism of PCR.
Expand YAC used in the field of Biotechnology.
