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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
विकल्प
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
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संबंधित प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
In vitro amplification of DNA or RNA segment is known as ___________.
Alu-I is obtained from which organism?
What is gene cloning? Explain different tools used for it.
Which of the following is TRUE regarding EcoR I?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the characterization techniques that are based on molecular weights of compounds.
From the following identify the methods of electrophoresis.
Human insulin is produced by culturing the genetically engineered ______
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Write steps involved in gene amplification.
Which one of the following enzymes is used to join DNA fragments?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
Which of the following best describes the relationship between genetic engineering and recombinant DNA?
