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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
विकल्प
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
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संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
The biological scissor is ______.
Polymerase chain reaction is most useful in ______.
What is PCR? Explain different steps involved in it.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Identify the CORRECT sequence of steps in PCR.
Which of the following device is required for PCR?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Which one is a true statement regarding DNA polymerase used in PCR?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which vector is mostly used in rDNA technology in plants?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
The polymerase chain reaction is a powerful technique to ______.
Write a short note on vectors.
What is EcoRI?
Expand YAC used in the field of Biotechnology.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
