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प्रश्न
'Restriction' in Restriction enzyme refers to ______.
विकल्प
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
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उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
How does a restriction nuclease function? Explain
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
How does one visualise DNA on an agarose gel?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
What is elution?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
