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प्रश्न
'Restriction' in Restriction enzyme refers to ______.
विकल्प
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
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उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
APPEARS IN
संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
How does a restriction nuclease function? Explain
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
