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प्रश्न
'Restriction' in Restriction enzyme refers to ______.
विकल्प
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
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उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
APPEARS IN
संबंधित प्रश्न
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
Give a reason why :
Single cloning site is preferred in a vector.
Which of the following radioisotope is not suitable for DNA labeling based studies?
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the principle involved in separation of DNA fragments using gel electrophoresis.
