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प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
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उत्तर
Exonuclease removes the nucleotides from the ends of the DNA chain while endonuclease cuts the DNA at the specific positions within the DNA strand.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
