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प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
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उत्तर
Exonuclease removes the nucleotides from the ends of the DNA chain while endonuclease cuts the DNA at the specific positions within the DNA strand.
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संबंधित प्रश्न
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
State the principle involved in separation of DNA fragments using gel electrophoresis.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
