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प्रश्न
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
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उत्तर
(a) No bands will be obtained as all DNA will be seen in the well only; DNA fragments being negatively charged will not move towards the negative end/cathode. DNA being negatively charged will remain stationed at the positive end/anode end of the agar block.
(b)
- The position of the positive terminal/end/anode and the negative terminal/end/cathode was inter-changed.
- The fragment with the least base pairs will get separated faster and move faster to the anode end.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
DNA fragments separate according to size through?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
'Restriction' in Restriction enzyme refers to ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How are DNA fragments visualised once they are separated by gel electrophoresis?
