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प्रश्न
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
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उत्तर
On finding the palindrome, the endonuclease binds to the DNA.
- It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and help in the formation of recombination DNA.
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संबंधित प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Distinguish between exonuclease and endonuclease.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
'Restriction' in Restriction enzyme refers to ______.
Which of the following statements does not hold true for restriction enzyme?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the principle involved in separation of DNA fragments using gel electrophoresis.
