हिंदी
कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

The role of DNA ligase in the construction of a recombinant DNA molecule is ______. - Biology

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प्रश्न

The role of DNA ligase in the construction of a recombinant DNA molecule is ______.

विकल्प

  • Formation of phosphodiester bond between two DNA fragments

  • Formation of hydrogen bonds between sticky ends of DNA fragments

  • Ligation of all purime and pyrimidine bases

  • None of the above

MCQ
रिक्त स्थान भरें
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उत्तर

The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments.

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Tools of Recombinant DNA Technology - Restriction Enzymes
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अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 13. | पृष्ठ ७७

संबंधित प्रश्न

Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?


Distinguish between exonuclease and endonuclease.


Give a reason why :
Single cloning site is preferred in a vector.


Which of the following radioisotope is not suitable for DNA labeling based studies?


DNA fragments separate according to size through?


DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______


A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______


Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?


In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.


What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?


Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?


A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.


How does one visualise DNA on an agarose gel?


Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing 

  1. Anode end
  2. smallest/lightest DNA strand in the matrix
  3. Agarose gel


State the importance of elution in this process.


State the principle involved in separation of DNA fragments using gel electrophoresis.


Identify the activity of endonuclease and exonuclease in the given image.


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