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प्रश्न
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
विकल्प
Formation of phosphodiester bond between two DNA fragments
Formation of hydrogen bonds between sticky ends of DNA fragments
Ligation of all purime and pyrimidine bases
None of the above
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उत्तर
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain briefly:
Restriction enzymes and DNA
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
