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प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
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उत्तर
Gel electrophoresis is a technique of separating DNA fragments formed by the action of restriction endonucleases.
The fragments of DNA are placed in a typical agarose gel under an electric field. The DNA fragments move towards the anode as these fragments are negatively charged molecules. The DNA fragments separate according to their size through the sieving effect provided by the agarose gel. The smaller the fragment size, the farther it moves. The separated DNA fragments are stained with ethidium bromide followed by exposure to ultraviolet radiation. The DNA fragments are seen as orangecoloured and are cut out from the agarose gel and extracted from the gel piece. This step is called elution.
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संबंधित प्रश्न
How does a restriction nuclease function? Explain
How does restriction endonuclease function?
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
'Restriction' in Restriction enzyme refers to ______.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
What is the precise definition of a palindromic sequence in DNA?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
