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प्रश्न
Distinguish between exonuclease and endonuclease.
Differentiate between exonuclease and endonuclease.
Discuss with your teacher and find out how to distinguish between exonuclease and endonuclease.
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उत्तर
| Sr. No. | Exonuclease | Endonuclease |
| 1. | It is a type of restriction enzyme that removes the nucleotide from the 5' or 3' ends of the DNA molecule. | It is a type of restriction enzyme that makes a cut within the DNA at a specific site to generate sticky ends. |
| 2. | They separated DNA base pairs at their terminal ends. |
They separate DNA at any location other than the terminal ends. |
| 3. | They work on single strands of DNA or gaps in double-stranded DNA. | They cut one or both strands of double-stranded DNA. |
APPEARS IN
संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain briefly:
Restriction enzymes and DNA
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following bacteria is not a source of restriction endonuclease?
Which of the following statements does not hold true for restriction enzyme?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
What is elution?
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
What is the precise definition of a palindromic sequence in DNA?
Which of the following was the very first restriction endonuclease to be isolated and characterized?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
How are separated DNA bands visualized following agarose gel electrophoresis?
