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प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
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उत्तर
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called 'sticky ends' on each strand.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
'Restriction' in Restriction enzyme refers to ______.
Which of the following statements does not hold true for restriction enzyme?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
