Advertisements
Advertisements
प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Advertisements
उत्तर
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called 'sticky ends' on each strand.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How does a restriction nuclease function? Explain
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the principle involved in separation of DNA fragments using gel electrophoresis.
