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प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
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उत्तर
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
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संबंधित प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
How does restriction endonuclease function?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
