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प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
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उत्तर
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in Restriction enzyme refers to ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Identify the activity of endonuclease and exonuclease in the given image.
