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प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
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उत्तर
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
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संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the following bacteria is not a source of restriction endonuclease?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Identify the activity of endonuclease and exonuclease in the given image.
