मराठी
कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.

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प्रश्न

In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.

पर्याय

  • Charge only

  • Size only

  • Charge to size ratio

  • All of the above

MCQ
रिकाम्या जागा भरा
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उत्तर

In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.

Explanation:

The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.

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पाठ 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७६]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 7. | पृष्ठ ७६

संबंधित प्रश्‍न

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.

Applying Chargaff’s rule, what conclusion can be drawn?


A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.


Molecular scissors, which cut DNA at specific site is ______.


DNA fragments separate according to size through?


Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?


While isolating DNA from bacteria, which of the following enzymes is not required?


Which of the following statements does not hold true for restriction enzyme?


Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?


A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.


A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?


CTTAAG
GAATTC

  1. What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
  2. What is their significance in biotechnology?

State the importance of elution in this process.


Identify the activity of endonuclease and exonuclease in the given image.


How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?


In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?


Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?


During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?


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