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प्रश्न
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
पर्याय
Charge only
Size only
Charge to size ratio
All of the above
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उत्तर
In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.
Explanation:
The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the principle involved in separation of DNA fragments using gel electrophoresis.
How are DNA fragments visualised once they are separated by gel electrophoresis?
